Oxytherm+多通道線粒體呼吸測定系統

先進的Clark氧電極系統——研究線粒體呼吸的科研利器

實時監測反應體系中溶解氧的濃度及變化，可多通道（最大8通道）同時檢測多個樣品

• 研究線粒體的I態、II態、III態、IV態呼吸，計算呼吸控制率及P/O比等；

• 研究肌肉、肝臟、大腦器官組織等細胞、細菌、酵母、藻類等微生物的呼吸（耗氧）速率；

• 研究呼吸途徑（抗氰呼吸、細胞色素氧化酶途徑、糖酵解途徑等）；

• 研究有氧參與的酶促反應(生物反應）速率，有氧參與的化學反應；

• 研究某些化學合成物質的放氧速率。

系統特點

• 24位高分辨率10^-3pmol·ml^-1

• 電子精確控溫(精度±0.5℃）

• 多通道實時顯示數據（1～8通道可選）

• 樣品用量少0.2～2.5ml

• 能與H⁺電極同時測定

氧電極用于測定酶活性Sevier C S，et al.Cell,2007,129(2):333-44

線粒體狀態呼吸和呼吸控制率

Papathanasiou S, Rickelt S, Soriano M E, et al. Tumor necrosis factor-α confers cardioprotection through ectopic expression of keratins K8 and K18[J]. Nature Medicine,2015,21(9):1076.

氧電極用于抗體免疫研究llani T, et al. Science, 2013, 341(6141):74 

氧電極用于測定細胞呼吸Zhang H, et al. Cancer Cell, 2007, 11(5):407

部分應用文獻

Cipolat S, Rudka T, Hartmann D, et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling[J]. Cell, 2006, 126(1):163-175.

Chen H, Vermulst M, Wang Y E, et al. Mitochondrial fusion is required for mtDNA stability in skeletal muscle and tolerance of mtDNA mutations[J]. Cell, 2010, 141(2): 280-289.

Wang G, Chen HW,Oktay Y, et al. PNPASE regulates RNA import into mitochondria[J]. Cell, 2010, 142(3): 456-467.

Lee H Y, Choi C S, Birkenfeld A L, et al. Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance[J]. Cell metabolism, 2010, 12(6): 668-674.

De Silva D, Fontanesi F, Barrientos A. The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit[J]. Cell metabolism, 2013, 18(5): 712-725.

Acetoze G, Weber K L, Ramsey J J, et al. Relationship between Liver Mitochondrial Respiration and Proton Leak in Low and High RFI Steers from Two Lineages of RFI Angus Bulls[J]. International Scholarly Research Notices, 2015, 2015.

Samaiya P K, Krishnamurthy S. Characterization of mitochondrial bioenergetics in neonatal anoxic model of rats[J]. Journal of bioenergetics and biomembranes, 2015, 47(3): 217-222.

Lai L, Jin J C, Xu Z Q, et al. Spectroscopic and Microscopic Studies on the Mechanism of Mitochondrial Toxicity Induced by CdTe ODs Modified with Different Ligands[J]. The Journal of membrane biology, 2015: 1一14.

Jacoby R P, MiIlarA H, Taylor N L. Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes[J]. Plant Mitochondria: Methods and Protocols, 2015: 165-185.

Mitochondrial Uncoupling and Glutathione Redox Cycling, with Protection Against Oxidant Injury in H9c2 Cells[J]. Food & Function, 2015, 6(2): 549-557.